4EKF
Structure of the Inactive Adenovirus Proteinase at 0.98 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-28 |
Detector | BRANDEIS 4K |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.270, 54.540, 42.410 |
Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.980 |
R-factor | 0.135 |
R-free | 0.16780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1NLN CHAIN A |
RMSD bond length | 0.029 |
RMSD bond angle | 0.045 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.000 |
High resolution limit [Å] | 0.980 | 0.980 |
Rmerge | 0.057 | 0.594 |
Number of reflections | 92231 | |
<I/σ(I)> | 25.4 | |
Completeness [%] | 99.1 | 97.1 |
Redundancy | 4.9 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 293 | 0.3M sodium citrate, pH 5.6, 0.8 M sodium acetate, 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |