4EK1
Crystal Structure of Electron-Spin Labeled Cytochrome P450cam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.020, 101.530, 72.980 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.970 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.492 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.303 | 47.303 | 2.070 |
| High resolution limit [Å] | 1.970 | 6.220 | 1.970 |
| Rmerge | 0.101 | 0.045 | 0.416 |
| Total number of observations | 6115 | 24179 | |
| Number of reflections | 54475 | ||
| <I/σ(I)> | 7.7 | 9.5 | 1.8 |
| Completeness [%] | 98.4 | 99.7 | 98.6 |
| Redundancy | 3.2 | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting-drop vapor diffusion | 7.4 | 279 | 12% PEG 8000, 0.1M Tris, pH 7.4, 0.2M KCl, 1mM camphor, sitting-drop vapor diffusion, temperature 279K |
