4EJW

Staphylococcus epidermidis TcaR in complex with streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	107.749, 107.749, 49.722
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.247
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kp7
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	8266
<I/σ(I)>	27.5	3.8
Completeness [%]	99.6	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1M Na-Hepes, pH7.5, 8-14% PEG 4000, 10-13% 2-propanol , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K