4EJS

Structure of yeast elongator subcomplex Elp456

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 2 3
格子定数 [Å]	186.513, 186.513, 186.513
格子定数 [度]	90.00, 90.00, 90.00

精密化法

分解能	39.765 - 2.606
R因子	0.1762
Rwork	0.174
R-free	0.22390
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	39.800	2.690
分解能 [Å] (高)	2.600	2.600
独立反射数	32701
完全性 [%]	99.2	99.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M Bis-tris propane, 0.8M DL-Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K