4EJS
Structure of yeast elongator subcomplex Elp456
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 186.513, 186.513, 186.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.765 - 2.606 |
| R-factor | 0.1762 |
| Rwork | 0.174 |
| R-free | 0.22390 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 32701 | |
| Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Bis-tris propane, 0.8M DL-Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
