4EJR
Crystal structure of major capsid protein S domain from rabbit hemorrhagic disease virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.734, 48.362, 65.163 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 49.350 - 2.000 |
| R-factor | 0.20257 |
| Rwork | 0.201 |
| R-free | 0.24064 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gh8 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.109 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.404 |
| Number of reflections | 24815 | |
| <I/σ(I)> | 25.7 | 3.97 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | 0.2 M magnesium chloride, 0.1 M HEPES sodium, 30% PEG400, pH 7.0, VAPOR DIFFUSION, temperature 289K |
