4EJ3
Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.776, 47.829, 129.178 |
Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
Resolution | 49.860 - 2.520 |
R-factor | 0.22079 |
Rwork | 0.218 |
R-free | 0.27693 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.151 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 38561 | |
<I/σ(I)> | 36.2 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.5M sodium formate, 0.1M Mes, 5% DMSO, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.5M sodium formate, 0.1M Mes, 5% DMSO, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |