4EIZ
Structure of Nb113 bound to apoDHFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.361, 77.517, 93.473 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 19.989 - 2.200 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.106 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 35739 | |
| Completeness [%] | 97.1 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 25.5% PEG4000, 0.085M NaCitrate, 0.17 M Ammonium Sulphate, 25% Glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
