4EIS
Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases (PMO-3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.740, 77.890, 82.050 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 35.740 - 1.370 |
| R-factor | 0.1176 |
| Rwork | 0.116 |
| R-free | 0.14920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.381 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.740 |
| High resolution limit [Å] | 1.370 |
| Number of reflections | 89009 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 8.5 | 277 | pH 8.5, SMALL TUBES, temperature 277K |
