4EIQ
Chromopyrrolic acid-soaked RebC-10x with bound 7-carboxy-K252c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.184, 77.703, 123.145 |
Unit cell angles | 90.00, 98.78, 90.00 |
Refinement procedure
Resolution | 47.300 - 2.760 |
Rwork | 0.206 |
R-free | 0.25510 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 2.790 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 28820 | |
Completeness [%] | 94.4 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM ammonium fluoride and 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |