4EIJ

Structure of the Mumps virus phosphoprotein oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	80.348, 80.348, 165.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.607 - 2.200
R-factor	0.1864
Rwork	0.181
R-free	0.23020
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.887
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.200	2.200
Rmerge		0.380
Number of reflections	10717
<I/σ(I)>	26.69	4.14
Completeness [%]	99.9	100
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1M sodium acetate pH 4.6, 2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K