4EIH
Crystal structure of Arg SH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.294, 81.845, 37.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.200 |
| R-factor | 0.16147 |
| Rwork | 0.160 |
| R-free | 0.18108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 25189 | |
| <I/σ(I)> | 29 | 5.27 |
| Completeness [%] | 95.1 | 69.3 |
| Redundancy | 6.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | pH8.5 100mM Tris, 15-17% PEG4000 0.2M Na Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
