4EI8
Crystal structure of Bacillus cereus TubZ, apo-form
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-12-04 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.753, 66.033, 58.350 |
Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
Rwork | 0.197 |
R-free | 0.24650 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.052 | 0.025 | 0.628 |
Number of reflections | 44373 | ||
<I/σ(I)> | 12.1 | ||
Completeness [%] | 99.9 | 98.9 | 99.6 |
Redundancy | 3.6 | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% PEG 4000, 0.1M Bis-Tris, 0.2M MgCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |