4EI8
Crystal structure of Bacillus cereus TubZ, apo-form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.753, 66.033, 58.350 |
| Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.197 |
| R-free | 0.24650 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.052 | 0.025 | 0.628 |
| Number of reflections | 44373 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 99.9 | 98.9 | 99.6 |
| Redundancy | 3.6 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% PEG 4000, 0.1M Bis-Tris, 0.2M MgCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
