4EI7
Crystal structure of Bacillus cereus TubZ, GDP-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-06-28 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.826, 76.015, 96.931 |
Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
Rwork | 0.185 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.059 | 0.027 | 0.464 |
Number of reflections | 52862 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 97.8 | 96.1 | 96.9 |
Redundancy | 5.2 | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 298 | 50% PEG 400, 0.1M MES, 1mM GTPgammaS, pH 5.5, VAPOR DIFFUSION, temperature 298K |