4EHV
Human p38 MAP kinase in complex with NP-F10 and RL87
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2011-10-31 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.830, 75.110, 77.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.600 |
R-factor | 0.2229 |
Rwork | 0.221 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.3) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.286 | 1.700 | |
High resolution limit [Å] | 1.600 | 10.000 | 1.600 |
Rmerge | 0.031 | 0.022 | 0.259 |
Number of reflections | 45792 | 219 | 5768 |
<I/σ(I)> | 23.78 | 57.62 | 3.37 |
Completeness [%] | 91.8 | 87.6 | 70.6 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 19-24% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |