4EHS

Crystal structure of Helicobacter pylori DnaG Primase C terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97860
Spacegroup name	P 21 21 21
Unit cell lengths	48.883, 61.455, 82.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 1.780
R-factor	0.19779
Rwork	0.195
R-free	0.24929
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	2.184
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (refmac_5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.780	4.830	1.780
Rmerge	0.067	0.035	0.332
Number of reflections	24493
<I/σ(I)>	42.3		3.8
Completeness [%]	99.5	99.9	94.2
Redundancy	6.7	6.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	PEG 5000 MME, NaCl, pH 7.4, vapor diffusion, hanging drop, temperature 289K