4EH6
Human p38 MAP kinase in complex with NP-F5 and RL87
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2011-07-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.120, 74.650, 77.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.100 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.333 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.200 | |
| High resolution limit [Å] | 2.100 | 10.000 | 2.100 |
| Rmerge | 0.036 | 0.015 | 0.299 |
| Number of reflections | 22558 | 247 | 2894 |
| <I/σ(I)> | 25.19 | 65.95 | 5.08 |
| Completeness [%] | 99.8 | 98 | 99.8 |
| Redundancy | 4.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 19-24% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
