4EGW
The structure of the soluble domain of CorA from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 61 |
| Unit cell lengths | 68.290, 68.290, 241.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.692 - 2.500 |
| R-factor | 0.1531 |
| Rwork | 0.153 |
| R-free | 0.19490 |
| Structure solution method | SAD+MR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 21977 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 28% PEG 8000, HEPES-MOPS, Alcohol mix, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
