4EGT
Crystal structure of major capsid protein P domain from rabbit hemorrhagic disease virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-21 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.99985 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.930, 88.144, 135.599 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.950 - 2.000 |
R-factor | 0.20073 |
Rwork | 0.199 |
R-free | 0.23154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gh8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.089 | 0.231 |
Number of reflections | 35882 | |
<I/σ(I)> | 29.8 | 8.3 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 289 | 0.1 M sodium acetate, 1.1 M succinic acid, 1.0% PEG2000 MME, pH 5.5, VAPOR DIFFUSION, temperature 289K |