4EGG
Computationally Designed Self-assembling tetrahedron protein, T310
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.760, 90.840, 107.700 |
| Unit cell angles | 90.00, 125.30, 90.00 |
Refinement procedure
| Resolution | 22.280 - 2.210 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dcl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.330 |
| High resolution limit [Å] | 2.210 | 4.850 | 2.250 |
| Rmerge | 0.106 | 0.090 | 0.553 |
| Number of reflections | 69236 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 96.8 | 97.2 | 93.4 |
| Redundancy | 6 | 8.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 7.5% polyethylene glycol 2000 monomethyl ether, 0.1 M sodium citrate, pH 6.0, 35% glycerol as cryoprotectant, vapor diffusion, hanging drop, temperature 298K |
