4EG9
1.9 Angstrom resolution crystal structure of Se-methionine hypothetical protein SAOUHSC_02783 from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Detector technology | CCD |
Collection date | 2012-03-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.346, 60.707, 94.156 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.890 - 1.900 |
R-factor | 0.21801 |
Rwork | 0.215 |
R-free | 0.27143 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.364 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.084 | 0.438 |
Number of reflections | 19583 | |
<I/σ(I)> | 16.4 | 3.1 |
Completeness [%] | 96.3 | 80.3 |
Redundancy | 4.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 7.0 mg/mL protein in 0.25 M sodium chloride, 0.01 M Tris, pH 8.3, screen: TRAP, H1, 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine, VAPOR DIFFUSION, SITTING DROP, temperature 295K |