4EFV

Crystal structure of OIF from Llama seminal plasma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97934
Spacegroup name	I 41
Unit cell lengths	108.930, 108.930, 49.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.834 - 2.320
R-factor	0.2053
Rwork	0.203
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bet
RMSD bond length	0.008
RMSD bond angle	1.176
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.500		2.380
High resolution limit [Å]	2.320	10.380	2.320
Rmerge	0.061	0.025	0.707
Number of reflections	12822	154	961
<I/σ(I)>	24.25	59.18	3.03
Completeness [%]	99.9	95.1	100
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop vapor diffusion	7.3	298	0.1M Tris-HCL pH 7.3, 0.7M LiSO4, 10% methanol, hanging drop vapor diffusion, temperature 298K