4EFQ
Bombyx mori lipoprotein 7 - platinum derivative at 1.94 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.071 |
Spacegroup name | P 1 |
Unit cell lengths | 41.806, 49.785, 54.958 |
Unit cell angles | 93.17, 94.87, 102.86 |
Refinement procedure
Resolution | 54.600 - 1.940 |
R-factor | 0.18675 |
Rwork | 0.185 |
R-free | 0.22665 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4efp |
RMSD bond length | 0.019 |
RMSD bond angle | 1.694 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | Auto-Rickshaw (MR protocol) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.600 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.068 | 0.153 |
Number of reflections | 29990 | |
<I/σ(I)> | 25.2 | 4.98 |
Completeness [%] | 94.2 | 75 |
Redundancy | 3.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES buffer, 22% PEG 3350, 200 mM KSCN, 2.0 mM K2PtCl6, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |