4EFL
Crystal structure of H-Ras WT in complex with GppNHp (state 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.666, 81.783, 121.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.350 - 1.900 |
| R-factor | 0.18524 |
| Rwork | 0.184 |
| R-free | 0.20433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.169 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.350 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.302 |
| Number of reflections | 13986 | |
| <I/σ(I)> | 7.8 | 2.4 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
