4EFI
Crystal Structure of 3-oxoacyl-(Acyl-carrier protein) Synthase from Burkholderia Xenovorans LB400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97740 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.377, 54.408, 60.836 |
| Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
| Resolution | 35.154 - 1.350 |
| R-factor | 0.1598 |
| Rwork | 0.159 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dfe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 3.210 | 1.300 |
| Rmerge | 0.035 | 0.610 | |
| Number of reflections | 78305 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.1 | 89.7 | 99.4 |
| Redundancy | 7.2 | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | Protein was from BuxeA.00171.a.A1.PW33644 set up at 26.35mg/ml, .4/.4 uL drops at 16c. Crystals were found in condition: 20% PEG 2000MME, 100mM Tris pH 8.5, 200mM Trimethylamine n-oxide cryoprotected with well solution +20% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
