4EFH
Acanthamoeba Actin complex with Spir domain D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.006, 71.791, 126.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.570 - 2.480 |
| R-factor | 0.1668 |
| Rwork | 0.165 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a5x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 5.390 | 2.480 |
| Rmerge | 0.141 | 0.087 | 0.491 |
| Number of reflections | 17452 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.5 | 96.9 | 100 |
| Redundancy | 4.4 | 4.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M NaSCN, 20% PEG3350, 0.1 M MgCl2, and 5 mM DTT, vapor diffusion, hanging drop, temperature 298K |
