4EFH
Acanthamoeba Actin complex with Spir domain D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.006, 71.791, 126.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.570 - 2.480 |
R-factor | 0.1668 |
Rwork | 0.165 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a5x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.590 |
High resolution limit [Å] | 2.480 | 5.390 | 2.480 |
Rmerge | 0.141 | 0.087 | 0.491 |
Number of reflections | 17452 | ||
<I/σ(I)> | 7.3 | ||
Completeness [%] | 99.5 | 96.9 | 100 |
Redundancy | 4.4 | 4.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M NaSCN, 20% PEG3350, 0.1 M MgCl2, and 5 mM DTT, vapor diffusion, hanging drop, temperature 298K |