4EFE
crystal structure of DNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.550, 86.530, 57.030 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| Rwork | 0.210 |
| R-free | 0.25420 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.070 |
| Number of reflections | 28245 |
| Completeness [%] | 96.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6 M Ammonium sulfate, 0.1M Na cacodelate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
