4EFA

Crystal Structure of the Heterotrimeric EGChead Peripheral Stalk Complex of the Yeast Vacuolar ATPase - second conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	P 2 21 21
Unit cell lengths	51.537, 93.648, 116.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.655 - 2.816
R-factor	0.2345
Rwork	0.230
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dl0
RMSD bond length	0.002
RMSD bond angle	0.499
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX (dev_1012)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.132	0.000
Number of reflections	14187
<I/σ(I)>	35.089	2.306
Completeness [%]	99.9	100
Redundancy	13.9	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292	0.1 M lithium sulfate, 0.1 M MES, 20% PEG mme 2000, 0.15 M glycine , pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 292K