4EF2
Crystal structure of a pheromone cOB1 precursor/lipoprotein, YaeC family (EF2496) from Enterococcus faecalis V583 at 2.10 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97966,0.97901 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.225, 75.691, 143.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.763 - 2.100 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.23220 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.858 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 29, 2011) |
Phasing software | SHELX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.763 | 2.170 | |
High resolution limit [Å] | 2.100 | 4.510 | 2.100 |
Rmerge | 0.040 | 0.017 | 0.746 |
Number of reflections | 28845 | 5557 | 3484 |
<I/σ(I)> | 12.37 | 37.1 | 1.4 |
Completeness [%] | 90.6 | 95.4 | 64.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 30.0% polyethylene glycol 400, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |