4EEW
Crystal structure of the Ubl domain of BAG6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9767 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.572, 43.193, 51.734 |
Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
Resolution | 32.720 - 1.300 |
R-factor | 0.18527 |
Rwork | 0.184 |
R-free | 0.19975 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.185 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.720 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 28619 | |
<I/σ(I)> | 25.9 | 3 |
Completeness [%] | 93.9 | 58.6 |
Redundancy | 2.3 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.2 M trimethylamine N-oxide, 0.1 M Tris, pH 8.5, 20% w/v PEG2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 294K |