4EEU
Crystal structure of phiLOV2.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Detector technology | CCD |
Collection date | 2010-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11611 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.967, 40.967, 130.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.478 - 1.407 |
R-factor | 0.2018 |
Rwork | 0.201 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ees |
RMSD bond length | 0.009 |
RMSD bond angle | 1.234 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.500 |
High resolution limit [Å] | 1.400 |
Number of reflections | 21959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 25% MPEG 2K, 0.2 M imidazole malate, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |