4EET
Crystal structure of iLOV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Detector technology | CCD |
Collection date | 2009-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115922 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.474, 46.474, 207.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.20162 |
Rwork | 0.201 |
R-free | 0.21146 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ees |
RMSD bond length | 0.007 |
RMSD bond angle | 1.157 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.200 |
Number of reflections | 72377 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 25% PEG 3350, 5% PEG 400, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |