4EET

Crystal structure of iLOV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Detector technology	CCD
Collection date	2009-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115922
Spacegroup name	P 41 21 2
Unit cell lengths	46.474, 46.474, 207.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.200
R-factor	0.20162
Rwork	0.201
R-free	0.21146
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ees
RMSD bond length	0.007
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.200
Number of reflections	72377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	25% PEG 3350, 5% PEG 400, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K