4EES
Crystal structure of iLOV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Detector technology | CCD |
| Collection date | 2008-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115922 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.736, 40.736, 123.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.805 |
| R-factor | 0.20793 |
| Rwork | 0.205 |
| R-free | 0.25656 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 10170 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 37.5% MPEG 2K, 0.2 M imidazole malate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
