4EER

Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2 C426A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	PIXEL
Collection date	2009-06-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 43 21 2
Unit cell lengths	41.023, 41.023, 123.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.753
R-factor	0.21843
Rwork	0.216
R-free	0.27541
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ees
RMSD bond length	0.018
RMSD bond angle	1.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.750
Number of reflections	9972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	37.5% MPEG 2K, 0.2 M imidazole malate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K