4EED
CorA coiled-coil mutant under Mg2+ presence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-27 |
Detector | MARMOSAIC CCD300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.700, 132.500, 179.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.790 - 3.920 |
R-factor | 0.2996 |
Rwork | 0.297 |
R-free | 0.35120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iub |
RMSD bond length | 0.011 |
RMSD bond angle | 1.235 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 4.020 |
High resolution limit [Å] | 3.920 | 3.920 |
Number of reflections | 21818 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 81.8 | 24.9 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 400, MgCl2, pH 7.0, temperature 298K, VAPOR DIFFUSION, HANGING DROP |