4EE8
Crystal structure of the Novel Phenazine Prenyltransferase EpzP (wildtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.690, 78.320, 83.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.610 - 1.930 |
| R-factor | 0.17464 |
| Rwork | 0.173 |
| R-free | 0.19845 |
| Structure solution method | PHASER |
| Starting model (for MR) | 2xlq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 30684 | |
| Completeness [%] | 98.6 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.4 M (NH4)2SO4, 100 mM HEPES/NaOH pH 7.5, 200 mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
