4EE6
Crystal Structure of the Novel Phenazine Prenyltransferase EpzP (methylated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.140, 135.600, 48.530 |
| Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
| Resolution | 29.930 - 1.330 |
| R-factor | 0.19088 |
| Rwork | 0.190 |
| R-free | 0.21313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.360 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 115868 | |
| Completeness [%] | 93.6 | 63.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 200 mM magnesium chloride, 30% (w/v) PEG4000, Tris hydrochloride ph 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
