4EE1
The structure of the S. aureus DnaG RNA Polymerase Domain bound to CTP and Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115 |
Spacegroup name | P 61 |
Unit cell lengths | 151.986, 151.986, 38.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.749 - 2.020 |
R-factor | 0.1838 |
Rwork | 0.182 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHENIX (AutoMR) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.749 | 49.745 | 2.130 |
High resolution limit [Å] | 2.020 | 6.390 | 2.020 |
Rmerge | 0.043 | 0.682 | |
Number of reflections | 32386 | ||
<I/σ(I)> | 14.8 | 14.4 | 1.1 |
Completeness [%] | 94.7 | 99.6 | 100 |
Redundancy | 11.4 | 10.8 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM CTP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |