4EDV
The structure of the S. aureus DnaG RNA Polymerase Domain bound to pppGpp and Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115 |
Spacegroup name | P 61 |
Unit cell lengths | 151.334, 151.334, 38.691 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.108 - 2.010 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.20320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (AutoMR) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 131.059 | 2.120 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.083 | 0.572 |
Number of reflections | 34211 | |
<I/σ(I)> | 17.8 | 1.3 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.1 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM pppGpp, 10 mM MnCl2, 1 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |