4EDT
The structure of the S. aureus DnaG RNA Polymerase Domain bound to ppGpp and Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115 |
| Spacegroup name | P 61 |
| Unit cell lengths | 152.164, 152.164, 38.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.926 - 2.005 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHENIX (AutoMR) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.405 | 2.110 |
| High resolution limit [Å] | 2.005 | 2.000 |
| Number of reflections | 34635 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 8.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM ppGpp, 10 mM MnCl2, 1 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
