4EDR

The structure of the S. aureus DnaG RNA Polymerase Domain bound to UTP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 61
Unit cell lengths	150.582, 150.582, 38.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.042 - 2.010
R-factor	0.1733
Rwork	0.172
R-free	0.20550
Structure solution method	PHENIX AutoMR
RMSD bond length	0.002
RMSD bond angle	0.718
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.630	38.630	2.120
High resolution limit [Å]	2.010	6.360	2.010
Number of reflections	33865
<I/σ(I)>	22.6	29.9	3.6
Completeness [%]	99.9	98.5	100
Redundancy	5.4	5.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM UTP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K