4EDM
Crystal structure of beta-parvin CH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.07810 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.360, 67.438, 105.712 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.680 | |
| Number of reflections | 23924 | |
| <I/σ(I)> | 25.891 | 1.905 |
| Completeness [%] | 98.2 | 92.5 |
| Redundancy | 7.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 298 | 12% PEG 550 MME, 0.1M Tris, pH 7.5, Microbatch, temperature 298K |
