4EDG
The structure of the S. aureus DnaG RNA Polymerase Domain bound to ATP and Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115 |
Spacegroup name | P 61 |
Unit cell lengths | 151.261, 151.261, 38.665 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.665 - 2.003 |
R-factor | 0.1633 |
Rwork | 0.162 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e2k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.062 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHENIX (AutoMR) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 130.996 | 43.665 | 2.110 |
High resolution limit [Å] | 2.003 | 6.330 | 2.000 |
Rmerge | 0.023 | 0.287 | |
Total number of observations | 5971 | 26029 | |
Number of reflections | 34499 | ||
<I/σ(I)> | 22.1 | 25 | 2.8 |
Completeness [%] | 99.8 | 98.9 | 98.8 |
Redundancy | 5.4 | 5.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM ATP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |