4EDB

Structures of monomeric hemagglutinin and its complex with an Fab fragment of a neutralizing antibody that binds to H1 subtype influenza viruses: molecular basis of infectivity of 2009 pandemic H1N1 influenza A viruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999
Spacegroup name	H 3 2
Unit cell lengths	217.115, 217.115, 266.016
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.340 - 2.500
R-factor	0.241
Rwork	0.239
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.136
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	83089
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	100mM Tris-HCl, 20% PEG 3350, 200mM calcium acetate, 8% 1,3-butanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K