4ED9
Crystal structure of a CAIB/BAIF family protein from Brucella suis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-09 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.400, 62.540, 70.310 |
Unit cell angles | 90.00, 117.81, 90.00 |
Refinement procedure
Resolution | 62.190 - 1.950 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21700 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.385 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.190 | 2.000 | |
High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
Rmerge | 0.063 | 0.022 | 0.217 |
Number of reflections | 25803 | 314 | 1785 |
<I/σ(I)> | 26.86 | 70.6 | 6.2 |
Completeness [%] | 98.0 | 96.9 | 92.3 |
Redundancy | 8.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 290 | Internal tracking number 230921. JCSG Core Suite III well A4. 0.1M CHES Buffer pH 9.5, 30.0% w/v PEG3,000, 20% ethylene glycol. BrsuA.18159.a.A1 pw34608 40mg/ml., vapor diffusion, sitting drop, temperature 290K |