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4ECZ

Human DNA polymerase eta - DNA ternary complex: AT crystal at pH 6.5 (Na+ MES) with 1 Ca2+ ion

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]95
Detector technologyCCD
Collection date2011-08-04
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1
Spacegroup nameP 61
Unit cell lengths98.786, 98.786, 82.431
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution25.440 - 1.834
R-factor0.1694
Rwork0.167
R-free0.20940
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.009
RMSD bond angle1.128
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Refinement softwarePHENIX ((phenix.refine: 1.6.1_357))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.860
High resolution limit [Å]1.8301.830
Number of reflections39319
<I/σ(I)>13.11.7
Completeness [%]98.497.5
Redundancy3.23.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP62930.1M MES, 6-17%(W/V) PEG 2K-MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.0

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