4ECH
Yeast Polyamine Oxidase FMS1, H67Q Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.160, 102.000, 75.830 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 39.377 - 2.400 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.380 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 47765 | |
| <I/σ(I)> | 15.7 | 2.7 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20% polyethylene glycol 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
