4EBR
Crystal structure of Autophagic E2, Atg10
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97951, 0.97966, 0.96 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 127.833, 127.833, 169.589 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.835 - 2.701 |
R-factor | 0.224 |
Rwork | 0.211 |
R-free | 0.25110 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.418 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.835 | 2.750 |
High resolution limit [Å] | 2.701 | 2.700 |
Number of reflections | 23007 | |
Completeness [%] | 98.6 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 50mM bis-Tris-HCl (pH 6.15), 3M NaCl, 1mM 1,4-diacetoxymercury-2,3-dimetoxybutane, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 50mM bis-Tris-HCl (pH 6.15), 3M NaCl, 1mM 1,4-diacetoxymercury-2,3-dimetoxybutane, VAPOR DIFFUSION, HANGING DROP, temperature 293K |