4EBQ

Fab structure of anti-Vaccinia virus D8L antigen mouse IgG2a LA5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.100002
Spacegroup name	P 21 21 21
Unit cell lengths	36.190, 93.360, 135.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.120 - 1.600
R-factor	0.1998
Rwork	0.198
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nsn
RMSD bond length	0.012
RMSD bond angle	1.467
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	135.960	31.120	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge		0.026	0.323
Total number of observations		11799	42438
Number of reflections	59711
<I/σ(I)>	17.7	21.6	2.4
Completeness [%]	96.6	97.5	93.8
Redundancy	5.5	5.6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	295	10% w/v PEG3000, 100 mM phosphate/citrate, 200 mM sodium chloride, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K