4EBG
Crystal structure of a DUF4467 family protein (SAV0303) from Staphylococcus aureus subsp. aureus Mu50 at 1.35 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.244, 35.058, 50.073 |
| Unit cell angles | 86.06, 79.66, 71.60 |
Refinement procedure
| Resolution | 17.689 - 1.200 |
| R-factor | 0.1379 |
| Rwork | 0.136 |
| R-free | 0.16730 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.581 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.689 | 17.689 | 1.230 |
| High resolution limit [Å] | 1.200 | 5.370 | 1.200 |
| Rmerge | 0.084 | 0.885 | |
| Total number of observations | 2514 | 11409 | |
| Number of reflections | 49882 | ||
| <I/σ(I)> | 6.7 | 7.1 | 0.7 |
| Completeness [%] | 77.9 | 91.7 | 67.4 |
| Redundancy | 3.7 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.20M ammonium dihydrogen phosphate, 20.00% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
