4EB5

A. fulgidus IscS-IscU complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Detector technology	CCD
Collection date	2011-02-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	76.411, 94.897, 150.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.280 - 2.530
R-factor	0.21193
Rwork	0.208
R-free	0.27924
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.604
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	80.280
High resolution limit [Å]	2.530
Number of reflections	35944
<I/σ(I)>	13.18
Completeness [%]	96.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		proteins 20 mg/mL (in buffer 13 mM Tris and 38 mM NaCl) mixed 1:1 with 0.2 M Ammonium acetate, HEPES 100 mM, PEG 3350 25%, DTT 5 mM, pH 7.5, VAPOR DIFFUSION, HANGING DROP